Human alpha1-microglobulin. Purification procedure, chemical and physiochemical properties.
نویسندگان
چکیده
منابع مشابه
Human a , - Microglobulin PURIFICATION PROCEDURE
A human cu,-glycoprotein of low molecular weight has been isolated from urine of patients with tubular proteinuria and shown to be present in serum and urine of healthy individuals. The isolation procedure includes ultrafiltration, gel chromatography, zone electrophoresis, and ion exchange chromatography. The yield of purified protein was 10 to 15% of the amount in concentrates of urinary prote...
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alpha1-Microglobulin (alpha1m) is a 26-kDa plasma and tissue glycoprotein. The protein has a heterogeneous yellow-brown chromophore consisting of small unidentified prosthetic groups localized to a free thiol group (C34) and three lysyl residues (K92, K118, and K130) around the entrance to a hydrophobic pocket. It was recently reported that alpha1m can bind heme and that a C-terminally processe...
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Hepatitis E virus (HEV), a plus-stranded RNA virus contains three open reading frames. Of these, ORF1 encodes the viral nonstructural polyprotein; ORF2 encodes the major capsid protein and ORF3 codes for a phosphoprotein of undefined function. Using the yeast two-hybrid system to screen a human cDNA liver library we have isolated, an N-terminal deleted protein, alpha(1) -microglobulin/bikunin p...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1977
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)40934-3